Biomaterial Database

Increased persistence of induced mutants of Chinese hamster cells by 12-O-tetradecanoylphorbol-13-acetate. 1986

W E Bradley, and F Laviolette

Hypoxanthine phosphoribosyltransferase deficient mutants of Chinese hamster ovary cells induced by ethyl methanesulfonate usually do not maintain their phenotype during growth in non-selective medium immediately following the induction. This phenomenon, called poor "persistence" of the induced mutation, is in most cases unrelated to growth rate but results from establishment of contact with wild type cells (Bradley, W. E. C. Exp. Cell Res., 129: 251, 1980). We report here that 12-O-tetradecanoylphorbol-13-acetate, a strong tumor promoter, increases the persistence of these mutants.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D010053	Ovary	The reproductive organ (GONADS) in female animals. In vertebrates, the ovary contains two functional parts: the OVARIAN FOLLICLE for the production of female germ cells (OOGENESIS); and the endocrine cells (GRANULOSA CELLS; THECA CELLS; and LUTEAL CELLS) for the production of ESTROGENS and PROGESTERONE.	Ovaries
D010704	Phorbols	The parent alcohol of the tumor promoting compounds from CROTON OIL (Croton tiglium).	Tigliane,Tiglianes
D011336	Probability	The study of chance processes or the relative frequency characterizing a chance process.	Probabilities
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003412	Cricetulus	A genus of the family Muridae consisting of eleven species. C. migratorius, the grey or Armenian hamster, and C. griseus, the Chinese hamster, are the two species used in biomedical research.	Hamsters, Armenian,Hamsters, Chinese,Hamsters, Grey,Armenian Hamster,Armenian Hamsters,Chinese Hamster,Chinese Hamsters,Grey Hamster,Grey Hamsters,Hamster, Armenian,Hamster, Chinese,Hamster, Grey
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005020	Ethyl Methanesulfonate	An antineoplastic agent with alkylating properties. It also acts as a mutagen by damaging DNA and is used experimentally for that effect.	Ethylmethane Sulfonate,Ethyl Mesilate,Ethyl Mesylate,Ethylmesilate,Ethylmesylate,Mesilate, Ethyl,Mesylate, Ethyl,Methanesulfonate, Ethyl,Sulfonate, Ethylmethane
D005260	Female		Females
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster