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We describe a two-site immunoradiometric assay for thyrotropin (TSH) in serum, based on use of two monoclonal antibodies directed against two separate antigenic determinants on the TSH molecule. One antibody is immobilized on polystyrene beads; the other is radioiodinated by a modified Chloramine T method. The detection limit of the assay is 0.02 milli-int. unit/L. The working range (CV less than 10%) is from 0.1 to greater than 50 milli-int. units/L. The log mean concentration of TSH in sera collected from 100 euthyroid subjects between 08:00 and 11:00 hours was 1.9 milli-int. units/L, the range 0.4-5.4 milli-int. units/L. Values for hyperthyroid patients and thyroid-cancer patients being treated with thyroxin were much lower than those for euthyroid persons. Results by this new assay correlated excellently with those by our conventional radioimmunoassay (r = 0.99) and also with a sensitive immunofluorometric TSH method (Delfia TSH) (r = 0.99).
T Helenius, and
S Tikanoja
November 1985,
Clinical chemistry,
T Helenius, and
S Tikanoja
October 1988,
Mayo Clinic proceedings,
T Helenius, and
S Tikanoja
August 1987,
Taiwan yi xue hui za zhi. Journal of the Formosan Medical Association,
T Helenius, and
S Tikanoja
March 1986,
Kaku igaku. The Japanese journal of nuclear medicine,
T Helenius, and
S Tikanoja
March 1987,
Kaku igaku. The Japanese journal of nuclear medicine,
T Helenius, and
S Tikanoja
October 1987,
Kaku igaku. The Japanese journal of nuclear medicine,
T Helenius, and
S Tikanoja
August 1987,
Clinical chemistry,
T Helenius, and
S Tikanoja
May 1986,
Annals of internal medicine,
T Helenius, and
S Tikanoja
March 1986,
Clinical chemistry,
T Helenius, and
S Tikanoja
September 1984,
Clinical chemistry,
