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A chemiluminescent labeled-antibody immunoassay has been developed for measurement of total thyroxin (T4) in serum. Monoclonal antibodies to T4 labeled with a chemiluminescent acridinium ester are used. Serum samples are incubated with the labeled antibodies and a thyroxin-rabbit immunoglobulin conjugate, then reacted with magnetizable particles containing sheep anti-rabbit immunoglobulin. The total reaction time is 40 min. The chemiluminescence intensity of the solid-phase immune complexes is inversely proportional to the concentration of T4 in the sample. The sensitivity of the assay is 1 nmol/L, and the working range of 20-190 nmol/L is characterized by CVs less than or equal to 10%.
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
October 2023,
BMC biotechnology,
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
January 1992,
Clinical chemistry,
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
October 1988,
Analytical biochemistry,
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
November 1985,
Clinical chemistry,
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
January 2009,
Methods in molecular biology (Clifton, N.J.),
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
January 1996,
Methods in molecular biology (Clifton, N.J.),
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
January 1992,
Clinical chemistry,
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
February 1983,
Clinical chemistry,
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
December 1988,
Clinical chemistry,
M L Sturgess, and
I Weeks, and
C N Mpoko, and
I Laing, and
J S Woodhead
January 2008,
Journal of immunoassay & immunochemistry,
