Biomaterial Database

Biosynthesis of lipid-linked oligosaccharides in embryonic liver. Glucosylation of mannose containing dolichyl diphosphate oligosaccharide. 1986

S V Kyosseva, and V I Zhivkov

A maximum rate of dolichyl phosphate [14C]glucose synthesis from 55-day embryos was achieved at 16 nM concentration of exogenous dolichyl phosphate and exceeded about 3 times that without addition of dolichyl phosphate. The highest values of [14C]glucose incorporation from UDP-[14C]glucose into dolichyl phosphate [14C]glucose, dolichyl diphosphate [14C]Glc-oligosaccharides and protein were reached at 5 min time point of incubation of liver microsomes both from embryos and sows. The radioactive incorporation into proteins was about 7-fold higher in liver microsomes from sows compared to that from embryos, probably due to the greater content of acceptor proteins in microsomes from sows. The enzymatic transfer of Glc3-oligosaccharide from a lipid carrier to endogenous protein acceptor in microsomes from pig embryonic and adult livers was considerably faster than the removal of glucose residues during the initial stages of processing of protein-bound oligosaccharides. One labelled compound was discovered in the CHCl3-CH3OH-H2O (1:1:0.3, by vol) extract after incubation of liver microsomes from embryos and sows with UDP-[14C]glucose. On the basis of its mobility on the chromatogram it appears to be GlcNAc2Man9Glc3.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008862	Microsomes, Liver	Closed vesicles of fragmented endoplasmic reticulum created when liver cells or tissue are disrupted by homogenization. They may be smooth or rough.	Liver Microsomes,Liver Microsome,Microsome, Liver
D011105	Polyisoprenyl Phosphate Sugars	Compounds functioning as activated glycosyl carriers in the biosynthesis of glycoproteins and glycophospholipids. They include the polyisoprenyl pyrophosphates.	Isoprenoid Phosphate Sugars,Sugar Pyrophosphorylpolyprenols,Phosphate Sugars, Isoprenoid,Phosphate Sugars, Polyisoprenyl,Pyrophosphorylpolyprenols, Sugar,Sugars, Isoprenoid Phosphate,Sugars, Polyisoprenyl Phosphate
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D002250	Carbon Radioisotopes	Unstable isotopes of carbon that decay or disintegrate emitting radiation. C atoms with atomic weights 10, 11, and 14-16 are radioactive carbon isotopes.	Radioisotopes, Carbon
D004287	Dolichol Monophosphate Mannose	A lipophilic glycosyl carrier of the monosaccharide mannose in the biosynthesis of oligosaccharide phospholipids and glycoproteins.	Dolichyl Mannopyranosyl Phosphate,Dolichyl Mannosyl Phosphate,Mannolipid,Mannosyl Phosphoryl Dolichol,D-Mannopyranosyldolichyl,Dolicholphosphate Mannose,Dolichyl Alpha-D-Mannopyranosyl Phosphate,Alpha-D-Mannopyranosyl Phosphate, Dolichyl,D Mannopyranosyldolichyl,Dolichol, Mannosyl Phosphoryl,Dolichyl Alpha D Mannopyranosyl Phosphate,Mannopyranosyl Phosphate, Dolichyl,Mannose, Dolichol Monophosphate,Mannose, Dolicholphosphate,Mannosyl Phosphate, Dolichyl,Monophosphate Mannose, Dolichol,Phosphate, Dolichyl Alpha-D-Mannopyranosyl,Phosphate, Dolichyl Mannopyranosyl,Phosphate, Dolichyl Mannosyl,Phosphoryl Dolichol, Mannosyl
D004622	Embryo, Mammalian	The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.	Embryonic Structures, Mammalian,Mammalian Embryo,Mammalian Embryo Structures,Mammalian Embryonic Structures,Embryo Structure, Mammalian,Embryo Structures, Mammalian,Embryonic Structure, Mammalian,Embryos, Mammalian,Mammalian Embryo Structure,Mammalian Embryonic Structure,Mammalian Embryos,Structure, Mammalian Embryo,Structure, Mammalian Embryonic,Structures, Mammalian Embryo,Structures, Mammalian Embryonic
D005260	Female		Females
D005865	Gestational Age	The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated from the onset of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization. It is also estimated to begin from fertilization, estrus, coitus, or artificial insemination.	Embryologic Age,Fetal Maturity, Chronologic,Chronologic Fetal Maturity,Fetal Age,Maturity, Chronologic Fetal,Age, Embryologic,Age, Fetal,Age, Gestational,Ages, Embryologic,Ages, Fetal,Ages, Gestational,Embryologic Ages,Fetal Ages,Gestational Ages
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose