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cis-8,11,14,17-[1-14C]Eicosatetraenoic acid was incubated with microsomes of ram seminal vesicles and 1 mM glutathione for 3 min at 37 degrees C. The main metabolite was identified as 17,18-dehydroprostaglandin E1 by capillary column gas chromatography-mass spectrometry. Human seminal fluid was analyzed for the presence of 17,18-dehydroprostaglandin E1 and prostaglandin E3. Whereas prostaglandin E3 could be demonstrated by capillary gas chromatography-mass spectrometry, 17,18-dehydroprostaglandin E1 could not be found under these conditions. However, human seminal fluid contained two compounds with a similar polarity on reversed phase high performance liquid chromatography as 17,18-dehydroprostaglandin E1 and prostaglandin E3. The two compounds were identified as 18,19-dehydroprostaglandin E1 and 18,19-dehydroprostaglandin E2 by gas chromatography-mass spectrometry, by UV analysis after conversion to the corresponding prostaglandin B compounds, and by ozonolysis. The amount of each of the two prostaglandins in human seminal fluid seemed to be in the same order of magnitude as the amount of prostaglandin E3.
E H Oliw, and
H Sprecher, and
M Hamberg
March 1975,
Science (New York, N.Y.),
E H Oliw, and
H Sprecher, and
M Hamberg
September 1982,
Prostaglandins,
E H Oliw, and
H Sprecher, and
M Hamberg
February 1985,
International journal of andrology,
E H Oliw, and
H Sprecher, and
M Hamberg
October 1987,
Journal of chromatography,
E H Oliw, and
H Sprecher, and
M Hamberg
January 1983,
Advances in prostaglandin, thromboxane, and leukotriene research,
E H Oliw, and
H Sprecher, and
M Hamberg
May 1984,
Nihon Sanka Fujinka Gakkai zasshi,
E H Oliw, and
H Sprecher, and
M Hamberg
December 1969,
Clinica chimica acta; international journal of clinical chemistry,
E H Oliw, and
H Sprecher, and
M Hamberg
January 1969,
International journal of fertility,
E H Oliw, and
H Sprecher, and
M Hamberg
September 1975,
Prostaglandins,
E H Oliw, and
H Sprecher, and
M Hamberg
October 1963,
The Journal of biological chemistry,
