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A method is described for the radioiodination of human calcitonin using the Iodogen reagent. The tracer was purified by 2 successive gel filtrations. The first, using Sephadex G-10, resulted in the complete removal of free 125iodide as assessed by ascending paper chromatography. The second, involving Sephadex G-75, permitted the separation of the tracer from high molecular weight aggregates. Analytical RP-HPLC showed a slight proportion (13%) of a contaminating product, probably (sulfoxyl-methionine9)calcitonin. The final yield, after radioiodination, was found to be 42.7 +/- 5.5% and the tracer displayed a specific activity of 850 +/- 110 Ci/mmol. The effects of long-term storage of the frozen tracer were studied.
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
March 1982,
Clinica chimica acta; international journal of clinical chemistry,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
May 2001,
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
January 1992,
Archives of andrology,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
January 1989,
International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
April 1990,
Nuclear medicine communications,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
January 2011,
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
December 1985,
Analytical biochemistry,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
August 1984,
Journal of neuroimmunology,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
January 1988,
International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology,
P Motté, and
P Vauzelle, and
G F Alberici, and
F Troalen, and
C Bohuon, and
D Bellet
February 1990,
Experimental and clinical endocrinology,
