Biomaterial Database

A retinal pigment epithelial cell-derived growth factor(s). 1986

J A Bryan, and P A Campochiaro

Proliferation of retinal pigment epithelial (RPE) cells, fibroblasts, and glial cells has been implicated in the pathogenesis of proliferative vitreoretinopathy. In this study, we demonstrate that RPE cells in culture produce a factor(s) that stimulates the growth of each of these cell types. After 48 hours of incubation with RPE-conditioned media, cell number is increased over that of controls by 110% for RPE cells, 105% for astrocytes, and 360% for corneal fibroblasts. Thymidine incorporation demonstrates that this increase is due to a stimulation of DNA synthesis. Preliminary characterization of the RPE growth-promoting activity demonstrates that it is heat stable, stable to extremes of pH, nondialyzable, and partially trypsin sensitive. These data suggest that RPE cells in culture produce a growth factor(s) targeted primarily at fibroblasts, but that can also stimulate their own growth and that of astrocytes.

UI	MeSH Term	Description	Entries
D010857	Pigment Epithelium of Eye	The layer of pigment-containing epithelial cells in the RETINA; the CILIARY BODY; and the IRIS in the eye.	Eye Pigment Epithelium
D012164	Retinal Diseases	Diseases involving the RETINA.	Disease, Retinal,Diseases, Retinal,Retinal Disease
D002452	Cell Count	The number of CELLS of a specific kind, usually measured per unit volume or area of sample.	Cell Density,Cell Number,Cell Counts,Cell Densities,Cell Numbers,Count, Cell,Counts, Cell,Densities, Cell,Density, Cell,Number, Cell,Numbers, Cell
D002455	Cell Division	The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.	M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D005128	Eye Diseases	Diseases affecting the eye.	Eye Disorders,Eye Disease,Eye Disorder
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D006133	Growth Substances	Signal molecules that are involved in the control of cell growth and differentiation.	Mitogens, Endogenous,Endogenous Mitogens