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Isolated intestinal mucosa cells of rats were used to investigate the intestinal transport of biotin. This method utilizing a double-label isotope technique showed that uptake could not be saturated, even in a wide range of biotin concentrations (0.01-2 microM). A metabolic inhibitor (antimycin A) did not prevent cell uptake of biotin. The transport mechanism was independent of temperature (Q10 = 1.04). When excess biotin was added to the incubation medium, there was no efflux of the vitamin from intestinal cells. The results also showed that the cells did not concentrate the vitamin, regardless of its concentration in the incubation medium. The mechanism of biotin uptake by rat cells at physiological concentrations is thus a passive diffusion phenomenon.
J Goré, and
C Hoinard, and
P Maingault
September 1983,
Journal of cellular physiology,
J Goré, and
C Hoinard, and
P Maingault
January 1985,
Annals of the New York Academy of Sciences,
J Goré, and
C Hoinard, and
P Maingault
July 1992,
The American journal of physiology,
J Goré, and
C Hoinard, and
P Maingault
April 1994,
Toxicology in vitro : an international journal published in association with BIBRA,
J Goré, and
C Hoinard, and
P Maingault
January 1970,
Acta physiologica Academiae Scientiarum Hungaricae,
J Goré, and
C Hoinard, and
P Maingault
December 1981,
Journal of applied physiology: respiratory, environmental and exercise physiology,
J Goré, and
C Hoinard, and
P Maingault
April 1987,
Life sciences,
J Goré, and
C Hoinard, and
P Maingault
February 1978,
Biochimica et biophysica acta,
J Goré, and
C Hoinard, and
P Maingault
April 1984,
Biochemical and biophysical research communications,
J Goré, and
C Hoinard, and
P Maingault
June 1983,
The Journal of nutrition,
