Biomaterial Database

[A rapid method for the isolation of Salmonellae from faeces (author's transl)]. 1978

G Ruffini, and L Santagata, and G Grechi

A rapid and simple method for detecting Salmonellae in faeces was developed. It was based on the motility of Salmonellae through a semi-solid medium in a U-tube. The migration of other intestinal bacteria was retarded by means of a magnesium salt concentration. 474 stool specimens were examined for Salmonellae in parallel with the usual isolation method. A total of 39 Salmonella strains were isolated, 37 (94.9%) by means of the selective migration procedure, 26 (66.7%) when the routine method was applied. Two strains of patients in chemo-therapy were missed by the rapid method, which was moreover inefficient in detecting S. typhi, due to its slow motility. However, the rapid method is recommended in addition to conventional mediums in screening of carriers for its higher frequency of positive isolations compared with multistep procedure (+27.3%).

UI	MeSH Term	Description	Entries
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D012475	Salmonella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.