The influence of a low and a high prepartum calcium (Ca) intake on Ca mobilization rate around parturition was studied in 44 dairy cows fed a ration sufficient for 1.90 times maintenance requirements during the dry period. The plasma Ca level declined on the day of parturition in the group fed the low Ca intake (LCa: 26.4 g/d) as well as in the group fed the high Ca intake (HCa: 87.2 g/d). Plasma Ca levels of the HCa group were lower at parturition, and in this group 1 cow had milk fever after parturition. In the HCa group 6 cows had a plasma Ca level less than or equal to 2.0 mmol/l at parturition and/or 10 h post-partum (pp), versus 1 cow in the LCa group. Na2EDTA was intravenously infused at 10 h pp to induce hypocalcaemia to a level of plasma Ca not bound to EDTA of approximately 1.0 mmol/l. The LCa groups tended to require more Na2EDTA than the HCa groups, however the difference was significant only in the younger cows. After the Na2EDTA infusion 7 cows of the HCa group and 1 of the LCa group did not recover spontaneously and needed to be treated. The mean plasma PTH levels of the LCa group ante-partum were slightly higher than those of the HCa group. The Ca level of the prepartum ration did not influence urinary hydroxyproline excretion, which suggests that the Ca intake of 26.4 g/d was too high to stimulate bone turnover. Comparison of the present results with those of an earlier experiment in which the prepartum Ca intake at a low feeding level (1.12 times maintenance) was studied, led to the conclusion that higher prepartum feed intake has a clear negative influence on Ca homeostasis around parturition.