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Protocol to measure glucose utilization in mouse tissues using radiolabeled 2-deoxy-D-glucose. 2024
Cyun-Ming Wu, and
Hao-Yun Li, and
Wen-Yi Li, and
Chih-Neng Hsu, and
Wei-Shun Yang, and
Gwo-Tsang Chuang, and
Tony Pan-Hou Che, and
Tung-Yuan Lee, and
Hsiao-Lin Lee, and
Siow-Wey Hee, and
Jiin-Horng Lee, and
Daniel Liao, and
Karen Chia-Wen Liao, and
Ya-Wen Liu, and
Chin-Feng Hsuan, and
Yi-Cheng Chang, and
Shao-Lun Chu
Department of Medicine, College of Medicine, National Taiwan University, Taipei 100, Taiwan.
2-deoxy-D-glucose (2DG) is a glucose analog converted to 2-deoxy-D-glucose-6-phosphate (2DG-6P) by hexokinase in glycolysis. While 2DG commonly measures glucose uptake, 2DG-6P detects glucose utilization. Here, we present a protocol to measure glucose utilization in various tissues after entering a mouse's body using radiolabeled 2DG. We describe steps for preparing mice and chemicals, extracting blood, adding chemicals, and dissolving tissue. We then detail procedures for calculating glucose utilization using the trapezoid rule.
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