[Evaluation of two miniaturized systems widely used in the laboratories for the identification of the "Enterobacteriaceae" (author's transl)]. 1979

G F Santini, and M Crovatto, and V Chiaradia, and M P Marchi

We have attended to a comparative research between two commercial microsystems: Enterotube and Minitek in order identify the Enterobacteriaceae and a reference system given by the combination of the usual macromethods already used in our laboratory. We have examined 401 bacterial cultures of Gram bacillus which we trought to belong to Enterobacteriums, coming from clinical material (excrements, urine, pharyngeal swabs, vaginal swabs, urethral swabs and espectoration) we have received for the bacteriological diagnosis. 390 of 401 cultures have shown to be Enterobacteriums. The biochemical reactions they have given show that the Enterotube and the Minitek have, with the usual system a good accordance for the following tests: dextrose (acid and gaz) lysin and ornithine decarboxylase, production of H2S and indole, phenylalanine deaminase and urease; while we have some statistically significant discordances for the fermenting of lactose and the use of citrate. We have also significant discordances E/C for the fermenting of dulcitole while the ones of Minitek are acceptables. The notes recommend the use, in the specialized bacteriological laboratories, of the conventional tests.

UI MeSH Term Description Entries
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004755 Enterobacteriaceae A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock. Coliform Bacilli,Enterobacteria,Ewingella,Leclercia,Paracolobactrum,Sodalis
D004756 Enterobacteriaceae Infections Infections with bacteria of the family ENTEROBACTERIACEAE. Enterobacterial Infections,Cronobacter Infections,Infections, Enterobacteriaceae,Infections, Enterobacterial,Cronobacter Infection,Enterobacteriaceae Infection,Enterobacterial Infection,Infection, Cronobacter,Infection, Enterobacteriaceae,Infection, Enterobacterial,Infections, Cronobacter
D001431 Bacteriological Techniques Techniques used in studying bacteria. Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic

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