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Alkaline borohydride reductive cleavage (beta-elimination) of glycophorin A isolated from one individual of the rare blood group Cad, resulted in the release of six acidic oligosaccharide-alditols which were separated by high-performance liquid chromatography (HPLC) on an alkyl amine silicagel column. The structure of four of them has been determined by the application of methanolysis, methylation analysis and 1H-NMR spectroscopy at 500 MHz. The structures and relative amounts were as follows: oligosaccharide 1: NeuAc(alpha 2-3)Gal(beta 1-3)GalNAc-ol (3.5%); oligosaccharide 3: GalNAc(beta 1-4)[NeuAc(alpha 2-3)]Gal(beta 1-3)GalNAc-ol (10.5%); oligosaccharide 5: NeuAc(alpha 2-3)Gal(beta 1-3)[NeuAc(alpha 2-6)]GalNAc-ol (10.4%); oligosaccharide 6: GalNAc(beta 1-4)[NeuAc(alpha 2-3)]Gal(beta 1-3)[NeuAc(alpha 2-6)]GalNAc-ol (71.2%). The two other oligosaccharides (2 and 4) were obtained in very low amount. The major pentasaccharide (oligosaccharide 6) carries the blood group Cad determinant and is a potent inhibitor of human anti-Sda antibody.
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
September 1981,
European journal of biochemistry,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
July 1981,
Biochimica et biophysica acta,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
September 1982,
European journal of biochemistry,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
April 1989,
Analytical biochemistry,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
November 1980,
FEBS letters,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
May 1986,
European journal of biochemistry,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
July 1990,
Bioorganicheskaia khimiia,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
November 1980,
FEBS letters,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
October 1988,
FEBS letters,
F Herkt, and
J P Parente, and
Y Leroy, and
B Fournet, and
D Blanchard, and
J P Cartron, and
H van Halbeek, and
J F Vliegenthart
September 1978,
FEBS letters,
