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alpha-Ketoglutarate dehydrogenase has been demonstrated for the first time in cell extracts from the filamentous fungus Aspergillus niger. A minimum protein concentration of 5 mg/ml is necessary for detecting enzyme activity, but a maximum of ca. 0.060 mumol/min per mg of protein is observed only when the protein concentration is above 9 mg/ml. alpha-Ketoglutarate can partly stabilize the enzyme against dilution in the assay system. Neither bovine serum albumin nor a variety of substrates or effectors of the enzyme could stabilize the enzyme against inactivation by dilution. A kinetic analysis of the enzyme revealed Michaelis-Menten kinetics with respect to alpha-ketoglutarate, coenzyme A, and NAD. Thiamine PPi was required for maximal activity. NADH, oxaloacetate, succinate, and cis-aconitate were found to inhibit the enzyme; AMP was without effect. Monovalent cations including NH4+ were inhibitory at high concentrations (greater than 20 mM). The highest enzyme activity was found in rapidly growing mycelia (glucose-NH4+ or glucose-peptone medium). We discuss the possibility that citric acid accumulation is caused by oxaloacetate and NADH inhibition of the alpha-ketoglutarate dehydrogenase of A. niger.
B Meixner-Monori, and
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A Habison, and
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Biochemistry,
B Meixner-Monori, and
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M Röhr
January 2000,
Pflugers Archiv : European journal of physiology,
B Meixner-Monori, and
C P Kubicek, and
A Habison, and
E M Kubicek-Pranz, and
M Röhr
July 2002,
European journal of biochemistry,
B Meixner-Monori, and
C P Kubicek, and
A Habison, and
E M Kubicek-Pranz, and
M Röhr
August 1988,
The Journal of biological chemistry,
B Meixner-Monori, and
C P Kubicek, and
A Habison, and
E M Kubicek-Pranz, and
M Röhr
December 1979,
Biochemistry,
B Meixner-Monori, and
C P Kubicek, and
A Habison, and
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M Röhr
April 2002,
Nihon rinsho. Japanese journal of clinical medicine,
B Meixner-Monori, and
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M Röhr
January 1999,
Annals of the New York Academy of Sciences,
B Meixner-Monori, and
C P Kubicek, and
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E M Kubicek-Pranz, and
M Röhr
February 1987,
Archives of microbiology,
B Meixner-Monori, and
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A Habison, and
E M Kubicek-Pranz, and
M Röhr
July 2024,
3 Biotech,
B Meixner-Monori, and
C P Kubicek, and
A Habison, and
E M Kubicek-Pranz, and
M Röhr
June 1979,
Biokhimiia (Moscow, Russia),
