-transparent.png){kind=logo}
Ten murine monoclonal antibodies have been produced that are specific for bovine milk lipoprotein lipase. One monoclonal antibody, bLPL-mAb-7, inhibited completely the apolipoprotein C-II (apo-C-II)-dependent enzymic hydrolysis of trioleoylglycerol in a phospholipid-stabilized emulsion, but had no effect on the hydrolysis of the water-soluble substrate p-nitro-phenylacetate. Four times more bLPL-mAb-7 was required to achieve 50% inactivation of lipoprotein lipase activity when the enzyme was preincubated with excess apo-C-II. Disruption of the binding of a dansyl-labeled apo-C-II peptide to lipoprotein lipase by bLPL-mAb-7 was demonstrated by resonance energy transfer, both in the presence and absence of lipid. This antibody thus appears to recognize the apo-C-II binding site of lipoprotein lipase. In addition, bLPL-mAb-7 also inhibited the lipoprotein lipase activity of human post-heparin plasma.
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
December 1998,
Journal of lipid research,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
August 1983,
Biochemistry,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
May 1985,
The Journal of biological chemistry,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
January 1985,
Comparative biochemistry and physiology. B, Comparative biochemistry,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
January 1982,
La Ricerca in clinica e in laboratorio,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
September 1995,
Journal of lipid research,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
June 2003,
The Journal of biological chemistry,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
October 1989,
The Journal of biological chemistry,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
July 1981,
The Journal of clinical investigation,
J C Voyta, and
D P Via, and
P K Kinnunen, and
J T Sparrow, and
A M Gotto, and
L C Smith
January 1996,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
