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Bovine pituitary fibroblast growth factor has been purified 222,000-fold to homogeneity by a combination of differential salt extraction, gel filtration, and ion exchange chromatography on Mono S column. Pituitary FGF is a single-chain polypeptide with an apparent molecular mass of 15,800 and an isoelectric point of 9.6. It is highly active in triggering the proliferation of bovine and human vascular endothelial cell [half-maximal stimulation at 23-40 pg/ml (1.5-2.6 pM) and saturation between 140 and 280 pg/ml (9.3-18.6 pM)]. It displays a similar activity on bovine vascular smooth muscle cells, corneal endothelial cells, granulosa and adrenal cortex cells, and rabbit costal chondrocytes.
D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
January 1996,
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D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
September 1984,
Proceedings of the National Academy of Sciences of the United States of America,
D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
January 1998,
Methods in molecular biology (Clifton, N.J.),
D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
June 1985,
FEBS letters,
D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
January 1990,
Computers in biology and medicine,
D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
January 1990,
Rinsho byori. The Japanese journal of clinical pathology,
D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
November 1984,
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D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
October 1986,
Biochimica et biophysica acta,
D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
January 1988,
Preparative biochemistry,
D Gospodarowicz, and
S Massoglia, and
J Cheng, and
G M Lui, and
P Böhlen
August 1984,
International journal of peptide and protein research,
