A high performance liquid chromatographic method is presented for the determination of chlophedianol hydrochloride in a complex tablet formulation. Tablets are dissolved in a water/acetonitrile (50/50) mixture. Samples are separated on an octadecylsilane column with a mobile phase of 70% acetonitrile and 30% aqueous solution of 0.005M 1-octanesulfonic acid, 1% acetic acid, and 1% diethylamine. Effluent is monitored by a spectrophotometric detector with a 254-nm filter. Chlophedianol is separated from a complex mixture of plant extracts and fillers in the tablets.