Biomaterial Database

Structural analysis of the gene coding for hepatitis B virus surface antigen and its product. 1985

H Takeshima, and J Inokoshi, and M Namiki, and J Shimada, and S Omura

The entire genome of hepatitis B virus (subtype adr) has been cloned into pBR322. The clones could be classified into at least seven groups by their restriction endonuclease cleavage maps. The nucleotide sequences of the hepatitis B surface antigen (HBsAg)-coding regions for five clones were determined and compared with published sequences of the HBsAg gene including those of adw, ayw, adyw and adr. The 13 available versions of the amino acid sequence of the polypeptide, predicted from the nucleotide sequences, were analysed in terms of the established specificities of the HBsAg, including the four subtypes. The analysis indicated that a relatively hydrophilic region of the HBsAg protein, spanning amino acid residues 110 to 160, specifies the major (w) and (r) subtype system. The (w/r) subtype appears to depend on changes in one or more variable amino acids at positions 47, 110, 113, 126 and 160 of the HBsAg polypeptide.

UI	MeSH Term	Description	Entries
D003062	Codon	A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).	Codon, Sense,Sense Codon,Codons,Codons, Sense,Sense Codons
D004279	DNA, Viral	Deoxyribonucleic acid that makes up the genetic material of viruses.	Viral DNA
D005814	Genes, Viral	The functional hereditary units of VIRUSES.	Viral Genes,Gene, Viral,Viral Gene
D006514	Hepatitis B Surface Antigens	Those hepatitis B antigens found on the surface of the Dane particle and on the 20 nm spherical and tubular particles. Several subspecificities of the surface antigen are known. These were formerly called the Australia antigen.	Australia Antigen,HBsAg,Hepatitis B Surface Antigen,Antigen, Australia
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein