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The covalent binding of benzo[a]pyrene (BP) to calf thymus DNA by brain microsomes isolated from control and 3-methylcholanthrene (3-MC) treated rats was investigated. The influence of incubation time, pH, and concentrations of protein, BP and NADPH on covalent binding was investigated to obtain optimum conditions for the in vitro binding of [3H]BP to DNA. Treatment of rats to 3-MC resulted in a 1.53-fold increase in the brain microsomal mediated covalent binding of [3H]BP to DNA. Inhibitors of monooxygenase enzyme activity such as alpha-naphthoflavone, metyrapone, 1-benzylimidazole and ellagic acid significantly inhibited the binding of [3H]BP to DNA from control and 3-MC stimulated brain microsomes. Our results indicate that inhibitors and inducers of monooxygenases may modulate brain enzyme-mediated binding of polycyclic aromatic hydrocarbons (PAHs) to DNA.
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
January 1982,
Carcinogenesis,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
November 1981,
Biochemical and biophysical research communications,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
October 1980,
Archives of biochemistry and biophysics,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
April 1976,
Proceedings of the National Academy of Sciences of the United States of America,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
May 1983,
Biochemical pharmacology,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
February 1976,
International journal of cancer,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
January 1983,
Carcinogenesis,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
May 1983,
Molecular pharmacology,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
March 1984,
Cancer letters,
M Das, and
S P Srivastava, and
P K Seth, and
H Mukhtar
June 1984,
Journal of biomolecular structure & dynamics,
