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A monospecific antibody against calf thymus DNA ligase composed of a single polypeptide with Mr = 130,000 cross-reacts with rodent and calf thymus DNA ligases. The antibody precipitates a single Mr = 200,000 polypeptide from detergent lysates of [3H] leucine-labeled mouse Ehrlich tumor cells and calf thymocytes. Pulse-chase experiments show that the Mr = 200,000 polypeptide in Ehrlich tumor cells has a half-life of about 0.5 h. In addition to the Mr = 200,000 polypeptide, a Mr = 130,000 polypeptide is detected in the partially purified enzyme preparations from radiolabeled Ehrlich tumor cells. These results suggest that DNA ligase is synthesized in mammalian cells as a Mr = 200,000 polypeptide and that the Mr = 200,000 polypeptide is degraded to a Mr = 130,000 polypeptide by a limited proteolysis in vitro.
H Teraoka, and
K Tsukada
July 1990,
The Journal of biological chemistry,
H Teraoka, and
K Tsukada
July 1990,
The Journal of biological chemistry,
H Teraoka, and
K Tsukada
December 1994,
The Journal of biological chemistry,
H Teraoka, and
K Tsukada
June 1984,
Nucleic acids research,
H Teraoka, and
K Tsukada
June 2002,
Radiation and environmental biophysics,
H Teraoka, and
K Tsukada
August 1995,
Molecular and cellular biology,
H Teraoka, and
K Tsukada
May 1997,
Mutation research,
H Teraoka, and
K Tsukada
January 1987,
European journal of biochemistry,
H Teraoka, and
K Tsukada
April 2014,
Cell reports,
H Teraoka, and
K Tsukada
August 1997,
Current biology : CB,
