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A simple method for quantitating proteins in the spots on two-dimensional polyacrylamide gel electropherograms is described. The system consists in three steps: (1) O'Farrell's two-dimensional gel electrophoresis of the proteins to be analysed; (2) staining of the gels with Coomassie brilliant blue; and (3) determination of the area and integrated density of the stained spots by the Joyce Loebl Magiscan-1 image analysis system. The method can be used for the determination of proteins in the range 0.5-100 micrograms/cm2; the amount of protein involved in most spots detected by the staining method actually falls within this range. As the minimum spot diameter that can easily be handled by the method is about 2 mm, as much as 30 ng of protein in such a spot can be determined. The method can also be applied to autoradiograms.
S Matsuhashi, and
T Mukai, and
K Hori
January 1993,
Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society,
S Matsuhashi, and
T Mukai, and
K Hori
August 1975,
FEBS letters,
S Matsuhashi, and
T Mukai, and
K Hori
January 1996,
Methods in enzymology,
S Matsuhashi, and
T Mukai, and
K Hori
January 1993,
Methods in molecular biology (Clifton, N.J.),
S Matsuhashi, and
T Mukai, and
K Hori
July 2000,
Electrophoresis,
S Matsuhashi, and
T Mukai, and
K Hori
October 1993,
The Journal of veterinary medical science,
S Matsuhashi, and
T Mukai, and
K Hori
May 1998,
Electrophoresis,
S Matsuhashi, and
T Mukai, and
K Hori
January 2003,
Advances in protein chemistry,
S Matsuhashi, and
T Mukai, and
K Hori
October 1997,
Electrophoresis,
S Matsuhashi, and
T Mukai, and
K Hori
October 1976,
Analytical biochemistry,
