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Isoenzymes of human red cell glutamate-pyruvate transaminase (GPT) were resolved by isoelectric focusing (IEF) of hemolysates in polyacrylamide gels at pH 5.0-7.0. The bands of enzyme activity required both alpha-ketoglutarate and L-alanine in the staining mixture for visualization, indicating that the bands were not lactate dehydrogenase or glutamate dehydrogenase. Phenotyping of 41 individuals by IEF, including types GPT 1, 2A, 1-2A, 1-2B, and 2A-2B, agreed with the typing results obtained by electrophoresis in starch gels and in polyacrylamide gels at acid and alkaline pH. Analysis of one kindred demonstrated autosomal codominant transmission of the rare GPT*2B gene through 3 generations. IEF facilitates phenotyping by permitting identification of the GPT types on a single gel with a considerable reduction in time and cost. Although no new variants were found in this investigation, IEF may be more powerful for the recognition of presently undetected variants of GPT.
C F Pelzer, and
R A Norum
September 1975,
Humangenetik,
C F Pelzer, and
R A Norum
June 1979,
Jinrui idengaku zasshi. The Japanese journal of human genetics,
C F Pelzer, and
R A Norum
January 1974,
Zeitschrift fur Rechtsmedizin. Journal of legal medicine,
C F Pelzer, and
R A Norum
January 1987,
Human heredity,
C F Pelzer, and
R A Norum
December 1987,
The Tohoku journal of experimental medicine,
C F Pelzer, and
R A Norum
January 1987,
Beitrage zur gerichtlichen Medizin,
C F Pelzer, and
R A Norum
January 1991,
Transfusion,
C F Pelzer, and
R A Norum
January 1983,
Human heredity,
C F Pelzer, and
R A Norum
July 1970,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
C F Pelzer, and
R A Norum
March 1997,
Genomics,
