Biomaterial Database

1-Naphthol conjugation in isolated cells from liver, jejunum, ileum, colon and kidney of the guinea pig. 1985

M Schwenk, and M Locher

Cells from liver, jejunum, ileum, colon and kidney of the guinea pig were freshly prepared by standard isolation procedures. Cells were incubated in the presence of several concentrations of 1(14C)-naphthol, and formation of 1-naphthol glucuronide and 1-naphthol sulphate was assessed at various times by thin layer chromatography. Cells from all five tissues conjugated 1-naphthol. The velocity of conjugation was fastest in jejunals cells (2.9 nmole/mg prot X min) followed by cells from liver, ileum, colon and kidney (0.2 nmole/mg prot X min). The apparent Km-values for glucuronidation ranged from 17 microM to 32 microM, and those for sulphation from 15 microM to 35 microM. Each tissue had a specific conjugation pattern. The two extremes were kidney cells, which had a glucuronidation/sulphation ratio in excess of 10, and colon cells which had a ratio of 0.38. The data suggest that these tissues possess different levels of 1-naphthol-conjugating enzymes, which resemble with regard to their apparent substrate affinities.

UI	MeSH Term	Description	Entries
D007583	Jejunum	The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum.	Jejunums
D007668	Kidney	Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.	Kidneys
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D009284	Naphthols	Naphthalene derivatives carrying one or more hydroxyl (-OH) groups at any ring position. They are often used in dyes and pigments, as antioxidants for rubber, fats, and oils, as insecticides, in pharmaceuticals, and in numerous other applications.	Hydroxynaphthalene,Hydroxynaphthalenes,Naphthol
D003106	Colon	The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.	Appendix Epiploica,Taenia Coli,Omental Appendices,Omental Appendix,Appendices, Omental,Appendix, Omental
D005965	Glucuronates	Derivatives of GLUCURONIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that include the 6-carboxy glucose structure.	Glucosiduronates,Glucuronic Acids,Acids, Glucuronic
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia