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Rates of hydrolysis of 3-hydroxybenzo[a]pyrenyl glucuronide by microsomal beta-glucuronidase from rat liver were 397 +/- 17 nmol/min per g protein and were half-maximal with about 100 microM substrate. Treatment of rats with phenobarbital or 3-methylcholanthrene, which elevates activities of glucuronosyltransferase(s), lowered rates of hydrolysis of benzo[a]pyrene glucuronide by 25%. Hydrolysis of the glucuronide by microsomal beta-glucuronidase was stimulated by micromolar concentrations of calcium in the range existing in cytosol of hepatocytes (apparent Km approximately 0.2 microM). Thus, humoral factors that change intracellular concentrations of free calcium may alter the production and export of glucuronides of benzo[a]pyrene metabolites from the liver.
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
January 1978,
Science (New York, N.Y.),
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
June 1984,
Biochemical and biophysical research communications,
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
February 2007,
Cancer gene therapy,
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
January 1995,
Journal of analytical toxicology,
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
January 1982,
Clinical chemistry,
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
March 1954,
Endocrinology,
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
March 1969,
The Biochemical journal,
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
January 1979,
Preparative biochemistry,
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
December 1997,
Biological & pharmaceutical bulletin,
M Whittaker, and
P M Sokolove, and
R G Thurman, and
F C Kauffman
September 1952,
Science (New York, N.Y.),
