-transparent.png){kind=logo}
Cyclic adenosine 3':5'-monophosphate (cAMP)-dependent and cAMP-independent protein kinase activity and the isoenzyme pattern of cAMP-dependent protein kinase were compared in tissues from human nonneoplastic gastric mucosa, 11 human gastric carcinomas, and 2 xenotransplantable human gastric carcinomas (SC-6-JCK and St-15). No difference in total protein kinase activity could be observed between nonneoplastic gastric mucosa and gastric carcinomas. According to diethylaminoethyl cellulose column chromatography, the isoenzyme pattern of the nonneoplastic gastric mucosa was the same in both the gastric fundus and the antrum, and the activity ratio of type II to type I was 5.01. In gastric carcinomas, the elevation of type I was detected independently of the histological type. In xenotransplantable human gastric carcinomas in nude mice, type I isoenzyme was significantly elevated. The activity ratio of type II to type I was 1.70 in SC-6-JCK carcinomas and 1.81 in St-15 carcinomas, respectively. These results suggest that type I cAMP-dependent protein kinase activity in the stomach may be a biochemical marker for malignant transformation and transplantability of gastric tumors.
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
December 1973,
The Journal of biological chemistry,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
January 1976,
Biochimica et biophysica acta,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
October 1972,
The Journal of biological chemistry,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
January 1991,
Acta physiologica Hungarica,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
December 1972,
The British journal of dermatology,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
April 1979,
Biochimica et biophysica acta,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
August 1977,
FEBS letters,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
January 1974,
Biochemical Society transactions,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
January 1976,
Biochemical Society transactions,
W Yasui, and
H Sumiyoshi, and
A Ochiai, and
M Yamahara, and
E Tahara
December 1973,
Canadian journal of physiology and pharmacology,
