Biomaterial Database

An automated analysis for superoxide dismutase enzyme activity. 1985

B H Gray, and L H Lee, and J F Wyman

The pyrogallol autoxidation assay for superoxide dismutase was adapted for analysis by centrifugal analyzer. The precision of the enzymatic assay was estimated using bovine blood superoxide dismutase. Specific activity determined for human erythrocyte superoxide dismutase by the procedure agreed with previously reported determinations. Recovery of bovine blood superoxide dismutase from hemolysates provided a measure of proportional analytic error. Difficulties inherent in attempting to spectrophotometrically assay an enzyme catalyzing a reaction requiring a free radical substrate cannot be eliminated by an automated method. However, the centrifugal analyzer method makes routine enzymatic analysis for superoxide dismutase in numerous samples technically feasible. Moreover, automated analysis can conveniently include a standard such as bovine blood superoxide dismutase when enzyme levels in tissue extracts are assayed. These advantages make the method for assay of superoxide dismutase enzyme activity a useful procedure.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D011748	Pyrogallol	A trihydroxybenzene or dihydroxy phenol that can be prepared by heating GALLIC ACID.	1,2,3-Trihydroxybenzene,Pyrogallic acid,acid, Pyrogallic
D002498	Centrifugation	Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
D004912	Erythrocytes	Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.	Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D013482	Superoxide Dismutase	An oxidoreductase that catalyzes the reaction between SUPEROXIDES and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide.	Hemocuprein,Ag-Zn Superoxide Dismutase,Cobalt Superoxide Dismutase,Cu-Superoxide Dismutase,Erythrocuprein,Fe-Superoxide Dismutase,Fe-Zn Superoxide Dismutase,Iron Superoxide Dismutase,Manganese Superoxide Dismutase,Mn-SOD,Mn-Superoxide Dismutase,Ag Zn Superoxide Dismutase,Cu Superoxide Dismutase,Dismutase, Ag-Zn Superoxide,Dismutase, Cobalt Superoxide,Dismutase, Cu-Superoxide,Dismutase, Fe-Superoxide,Dismutase, Fe-Zn Superoxide,Dismutase, Iron Superoxide,Dismutase, Manganese Superoxide,Dismutase, Mn-Superoxide,Dismutase, Superoxide,Fe Superoxide Dismutase,Fe Zn Superoxide Dismutase,Mn SOD,Mn Superoxide Dismutase,Superoxide Dismutase, Ag-Zn,Superoxide Dismutase, Cobalt,Superoxide Dismutase, Fe-Zn,Superoxide Dismutase, Iron,Superoxide Dismutase, Manganese