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Insoluble extracts of human bronchogenic squamous cell carcinoma were solubilized by desoxycholate. After fractionation, the soluble extracts were used to prepare antisera in guinea pigs. These antisera, after being highly absorbed with homogenates of several normal organ, serum and carcinoembryonic antigen, were reactive only with squamous cell carcinoma. There was no reactivity to fetal lung, normal adult organs or other cancers.
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