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The growth of the established malignant HeLa cells was much more inhibited by mechlorethamine (HN2) than that of the nonmalignant 3T3 cells. An initial growth inhibition was followed by complete inhibition even if HN2 was removed from the culture medium. Significant cell death did not occur during the first 24 h of HN2 treatment, but well later on. Incubation with 2.5 micrograms/ml HN2 caused increased release of lysosomal enzymes into the medium and decreased intracellular enzyme activities after a latency period of at least 6 h. The lysosomes from HeLa cells did not seem to be more fragile than those from 3T3 cells. Incubation with 0.25 micrograms/ml HN2 did not alter the intra- or extracellular enzyme levels the first 24 h, but well later on even after refeeding with medium without HN2. This study indicates that lysosomal enzymes are not involved in the genesis of the process leading to cell inactivation by HN2, but may well be correlated with the cytotoxic effects leading to cell death.
G Kavli, and
O K Andersen, and
G Volden
December 1979,
Biomedicine / [publiee pour l'A.A.I.C.I.G.],
G Kavli, and
O K Andersen, and
G Volden
July 1984,
The British journal of dermatology,
G Kavli, and
O K Andersen, and
G Volden
June 1974,
The Journal of experimental medicine,
G Kavli, and
O K Andersen, and
G Volden
September 1982,
Arerugi = [Allergy],
G Kavli, and
O K Andersen, and
G Volden
January 1977,
Cytobios,
G Kavli, and
O K Andersen, and
G Volden
March 1973,
Proceedings of the National Academy of Sciences of the United States of America,
G Kavli, and
O K Andersen, and
G Volden
November 1977,
British journal of pharmacology,
G Kavli, and
O K Andersen, and
G Volden
December 1974,
Journal of immunology (Baltimore, Md. : 1950),
G Kavli, and
O K Andersen, and
G Volden
May 1975,
The Journal of pharmacology and experimental therapeutics,
G Kavli, and
O K Andersen, and
G Volden
January 1989,
Zeitschrift fur Rheumatologie,
