-transparent.png){kind=logo}
Cell surface glycosaminoglycans (GAGs) were measured, after various treatments, by their binding to Acridine Orange using flow cytometry. Using a critical electrolyte concentration and combining it with specific degradation of individual GAG elements, it was found possible to differentiate between GAG components. The technique was adapted for electron microscopy level to reveal characteristics of membrane-associated GAG. By this means, the cell membrane of the human leukaemic cell line K562 was shown to contain a large amount of GAG; 75% of it was highly sulphated GAG, mostly heparan sulphate. This component was evenly distributed in the outer plasma membrane layer. In the presence of other GAGs, the appearance of complex proteoglycan granules was detected.
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
November 2018,
Scientific reports,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
August 1990,
The American journal of pathology,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
January 1989,
Basic and applied histochemistry,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
May 1998,
Mutation research,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
May 1998,
Mutation research,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
July 2003,
Mutation research,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
November 1978,
The Biochemical journal,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
January 1997,
Molecular human reproduction,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
February 1992,
Journal of the Formosan Medical Association = Taiwan yi zhi,
J Timar, and
F Boldog, and
L Kopper, and
K Lapis
May 1988,
Cytometry,
