-transparent.png){kind=logo}
Radioactive- and stable isotope-containing substrates were used to identify the biosynthetic precursors of the beta-lactam antibiotic, thienamycin, in Streptomyces cattleya. Acetate is utilized by the organism to form C(6) and C(7) of the beta-lactam ring. The two carbons of the hydroxyethyl group attached to C(6) are both derived from the methyl of methionine. The cysteaminyl side chain attached to C(2) is derived from cysteine. Selective inhibition of thienamycin and cephamycin C biosynthesis has been achieved either through the addition of metabolic inhibitors or through manipulation of the growth medium. These results suggest that the two beta-lactam antibiotics, thienamycin and cephamycin C, are formed by different biosynthetic pathways.
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
April 2003,
Chemistry & biology,
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
January 1993,
Journal of industrial microbiology,
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
January 1993,
Chinese journal of biotechnology,
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
October 1983,
Biotechnology and bioengineering,
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
April 2011,
Antimicrobial agents and chemotherapy,
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
September 1983,
The Journal of antibiotics,
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
June 1995,
Microbiology (Reading, England),
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
March 2010,
The Journal of antibiotics,
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
April 1979,
Antimicrobial agents and chemotherapy,
J M Williamson, and
E Inamine, and
K E Wilson, and
A W Douglas, and
J M Liesch, and
G Albers-Schönberg
January 1995,
Antonie van Leeuwenhoek,
