The kidney and urine glycosphingolipids of five pigmentation mutants which are known to have altered secretion of kidney lysosomal enzymes were examined. Among 34 pigmentation mutants which have been studied (Novak, E. K., Wieland, F., Jahreis, G. P., and Swank, R. T. (1980) Biochem. Genet. 18, 549-561) eight are known to have a 1.5- to 2.5-fold increase in kidney beta-glucuronidase in testosterone-treated females. These mutants appear to have defects in lysosomal processing, and because the mutations are at separate loci, each mutant probably affects different steps in assembly and/or exocytosis of lysosomes and related subcellular organelles. To test whether the neutral glycosphingolipids, galabiglycosylceramides, and globotriglycosylceramides thought to be associated with kidney lysosomes (McCluer, R. H., Williams, M. A., Gross, S. K., and Meisler, M. H. (1981) J. Biol. Chem. 256, 13112-13120) also exhibit abnormal secretion in the mutants with lysosomal enzyme abnormalities, the mutants beige-J, pale ear, light ear, pallid, and ruby eye-2-J were studied. The kidney and urine neutral glycosphingolipids from males of each mutant and C57BL/6J control mice were analyzed by high performance liquid chromatography. Beige-J, light ear, and pale ear showed marked increases in total kidney glycolipids; globotriglycosylceramides accounted for the bulk of the increase. Ruby eye-2-J showed less marked but significantly increased quantities of one galabiglycosylceramide and the globotriglycosylceramides in kidney. Pallid showed no significant increase in total kidney glycolipids but the globotriglycosylceramides appeared slightly elevated. In terms of the decrease in total urinary glycosphingolipids, the mutants fell into 2 categories. Beige-J, light ear, and pale ear were severely affected, whereas ruby eye-2-J and pallid were affected to a much lesser extent. Within the most severely affected group the excretion of the globotriglycosylceramides was more severely affected than that of the galabiglycosylceramides. The galabiglycosylceramides and globotriglycosylceramides appear to be specific markers of lysosomal membranes, but the independent behavior of these two classes of lipids during testosterone induction in normal mice and the differential effects on their secretion by different mutants indicate that they do not always exist in a characteristic ratio in a single type of subcellular organelle. All of the mutants accumulate organelles in their kidney proximal tubules which have distinct morphological characteristics as seen by electron microscopy.