Biomaterial Database

Catalase and superoxide dismutase activities in virulent and nonvirulent Staphylococcus aureus isolates. 1985

H Kanafani, and S E Martin

Catalase and superoxide dismutase (SOD) activities of virulent and nonvirulent isolates of Staphylococcus aureus were compared. The mean value of catalase activity for intact cell suspensions was 2,773 +/- 1,049 Kat f units (Kat f is defined as the ratio of the velocity constant of catalase at 0 min to the protein content in grams per milliliter); that of nonvirulent isolates was 154 +/- 92 Kat f units. The mean value of the catalase activities for lysates of virulent isolates was 260 +/- 120 Kat f units, and that of nonvirulent isolates was 31 +/- 19 Kat f units. Catalase levels in intact cells as well as in cell lysates were significantly different for virulent than for nonvirulent S. aureus isolates (P less than 0.001). The mean value of SOD activities was 20.85 +/- 11.48 U (1 U is defined as the amount of SOD required to inhibit the rate of reduction of cytochrome c by 50%) for virulent cell lysates, compared with a mean of 5.39 +/- 2.89 U for nonvirulent cell lysates. The SOD levels in virulent and nonvirulent isolates were significantly different (P less than 0.001). The virulence of the S. aureus isolates was determined by comparing weight gains of neonatal mice injected with virulent or nonvirulent strains. The percent weight gain of neonatal mice injected with virulent isolates was significantly lower than that of those injected with nonvirulent isolates.

UI	MeSH Term	Description	Entries
D001835	Body Weight	The mass or quantity of heaviness of an individual. It is expressed by units of pounds or kilograms.	Body Weights,Weight, Body,Weights, Body
D002374	Catalase	An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.	Catalase A,Catalase T,Manganese Catalase,Mn Catalase
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013211	Staphylococcus aureus	Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D013482	Superoxide Dismutase	An oxidoreductase that catalyzes the reaction between SUPEROXIDES and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide.	Hemocuprein,Ag-Zn Superoxide Dismutase,Cobalt Superoxide Dismutase,Cu-Superoxide Dismutase,Erythrocuprein,Fe-Superoxide Dismutase,Fe-Zn Superoxide Dismutase,Iron Superoxide Dismutase,Manganese Superoxide Dismutase,Mn-SOD,Mn-Superoxide Dismutase,Ag Zn Superoxide Dismutase,Cu Superoxide Dismutase,Dismutase, Ag-Zn Superoxide,Dismutase, Cobalt Superoxide,Dismutase, Cu-Superoxide,Dismutase, Fe-Superoxide,Dismutase, Fe-Zn Superoxide,Dismutase, Iron Superoxide,Dismutase, Manganese Superoxide,Dismutase, Mn-Superoxide,Dismutase, Superoxide,Fe Superoxide Dismutase,Fe Zn Superoxide Dismutase,Mn SOD,Mn Superoxide Dismutase,Superoxide Dismutase, Ag-Zn,Superoxide Dismutase, Cobalt,Superoxide Dismutase, Fe-Zn,Superoxide Dismutase, Iron,Superoxide Dismutase, Manganese
D014774	Virulence	The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.	Pathogenicity
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus