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The ability of erythromycin A base to penetrate and accumulate in tissue culture cells of human origin was investigated. The antibiotic was highly concentrated by early passage cells of normal bronchus, kidney, liver, lung, and skin and by cancer cells derived from breast, liver, and lung. Intracellular levels 4 to 12 times that of the extracellular milieu were obtained in both early-passage and transformed cells. The total quantity of erythromycin accumulated depended on the extracellular concentration of antibiotic, but the cellular/extracellular ratios were, for the most part, independent of the initial extracellular drug concentration. In all cell types tested, the accumulated antibiotic rapidly egressed when cells were incubated in antibiotic-free medium. Bioactivity assays demonstrated that the expelled drug was unmetabolized, fully active antibiotic. The concentration of erythromycin by a variety of human cell types probably accounts, in part, for the effectiveness of the antibiotic against intracellular parasites such as Legionella and Chlamydia spp.
J R Martin, and
P Johnson, and
M F Miller
November 1959,
Nature,
J R Martin, and
P Johnson, and
M F Miller
August 2005,
The Journal of parasitology,
J R Martin, and
P Johnson, and
M F Miller
January 1967,
The Wistar Institute symposium monograph,
J R Martin, and
P Johnson, and
M F Miller
December 1976,
Brain research,
J R Martin, and
P Johnson, and
M F Miller
December 1971,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
J R Martin, and
P Johnson, and
M F Miller
February 1984,
Kidney international,
J R Martin, and
P Johnson, and
M F Miller
September 1977,
The American journal of pathology,
J R Martin, and
P Johnson, and
M F Miller
May 1970,
Experimental cell research,
J R Martin, and
P Johnson, and
M F Miller
July 1974,
Experimental cell research,
J R Martin, and
P Johnson, and
M F Miller
January 1976,
Annals of the New York Academy of Sciences,
