-transparent.png){kind=logo}
The proallatocidin precocene II (6,7-dimethoxy-2,2-dimethyl-2H-benzo-[b]pyran) has previously been shown to induce centrolobular liver necrosis. Here we have examined the ability of precocene II to produce DNA damage in suspensions of freshly isolated rat hepatocytes using the alkaline elution technique with N-methyl-N'-nitro-N-nitrosoguanidine as a positive control. At concentrations (10(-4)-10(-5) M) which did not induce cytotoxicity as judged by the leakage of glutamic-oxaloacetic transaminase, precocene II was capable of producing DNA single-strand breaks. In addition, a dose-dependent DNA repair synthesis (unscheduled DNA synthesis, UDS) was detected in hepatocytes exposed to precocene II. The induction of UDS was measured by incorporation of [3H]thymidine into purified hepatic DNA via a membrane filter retention method and liquid scintillation counting. Hence, results obtained in the present study indicate the potential genotoxicity of precocene II and the utility of DNA damage and repair assays in genetic toxicology.
M T Hsia, and
B L Kreamer, and
S K Duddy
January 1993,
Biological trace element research,
M T Hsia, and
B L Kreamer, and
S K Duddy
August 1981,
Cancer letters,
M T Hsia, and
B L Kreamer, and
S K Duddy
November 1990,
Mutation research,
M T Hsia, and
B L Kreamer, and
S K Duddy
January 1982,
Radiobiologiia,
M T Hsia, and
B L Kreamer, and
S K Duddy
December 1996,
Toxicology in vitro : an international journal published in association with BIBRA,
M T Hsia, and
B L Kreamer, and
S K Duddy
July 1987,
Cancer letters,
M T Hsia, and
B L Kreamer, and
S K Duddy
September 1983,
The Biochemical journal,
M T Hsia, and
B L Kreamer, and
S K Duddy
January 1988,
Environmental and molecular mutagenesis,
M T Hsia, and
B L Kreamer, and
S K Duddy
January 1978,
Journal of nutritional science and vitaminology,
M T Hsia, and
B L Kreamer, and
S K Duddy
October 1983,
Mutation research,
