The distribution of anterogradely-transported horseradish peroxidase (HRP) was examined in the rostral mesencephalon and thalamus of cats and raccoons that had received injections of HRP in the cervical and/or lumbosacral enlargements of the spinal cord. Labeling was consistently observed in a large number of loci. All regions previously identified as targets of spinomesencephalic or spinothalamic fibers were included. Evidence of topographical organization was obtained in several regions. Adjacent fields of labeling were often separable on the basis of the distribution, appearance and topographical organization of the labeling. Subject to the methodological constraints imposed by the possibilities of transneuronal and/or collateral labeling, we conclude that a wide variety of loci in the thalamus receive direct spinal input. The organization of these projections suggests that each terminal region may be associated with different aspects of spinal cord function.