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The homogeneous enzyme immunoassay (EMIT) and radioimmunoassay (RIA) procedures for the quantitation of theophylline concentrations in serum were evaluated and compared. Both methods exhibited curves linear over the therapeutic range (range of concentration, 2.0-33.0 microgram/ml). Precision was acceptable for both methods with the coefficient of variation being less than 8.3%. The RIA procedure was found to be slightly more precise. The correlations between the EMIT and RIA procedures was found to be sufficient to allow the procedures to be used interchangeably. This would facilitate the test availability on a 24 hr, 7 day basis.
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