Biomaterial Database

Transport of glycyl-L-proline by mouse intestinal brush-border membrane vesicles. 1985

V M Rajendran, and A Berteloot, and K Ramaswamy

The characteristics of [14C]glycyl-L-proline transport have been studied using brush-border membrane vesicles from mouse small intestine in order to investigate the transport of nonhydrolyzable peptide across the brush-border membrane. Uptake curves for the peptide did not exhibit overshoot phenomena and were similar under Na+ or K+ gradient conditions (extravesicular greater than intravesicular). However, L-proline was transported by Na+ gradient-dependent system. Analysis of the incubation medium and the intravesicular contents showed that there was negligible hydrolysis of the peptide. Transport of glycyl-L-proline was saturable, conforming to Michaelis-Menten kinetics with a Km of 30.8 +/- 1.9 mM and a Vmax of 5.96 +/- 0.17 nmol.mg prot-1.0.4 min-1. Uptake of glycyl-L-proline was not significantly inhibited by free amino acids nor by most of the peptides containing D amino acids but was strongly inhibited (up to 64%) by various di- and tripeptides of L amino acids. These results clearly show that glycyl-L-proline was transported by a Na+-independent, carrier-mediated process. Our results suggest that the nonhydrolyzable peptides are transported mostly by carrier-mediated processes in contrast to hydrolyzable peptides.

UI	MeSH Term	Description	Entries
D007421	Intestine, Small	The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.	Small Intestine,Intestines, Small,Small Intestines
D008810	Mice, Inbred C57BL	One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases.	Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D008871	Microvilli	Minute projections of cell membranes which greatly increase the surface area of the cell.	Brush Border,Striated Border,Border, Brush,Border, Striated,Borders, Brush,Borders, Striated,Brush Borders,Microvillus,Striated Borders
D002352	Carrier Proteins	Proteins that bind or transport specific substances in the blood, within the cell, or across cell membranes.	Binding Proteins,Carrier Protein,Transport Protein,Transport Proteins,Binding Protein,Protein, Carrier,Proteins, Carrier
D002462	Cell Membrane	The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.	Plasma Membrane,Cytoplasmic Membrane,Cell Membranes,Cytoplasmic Membranes,Membrane, Cell,Membrane, Cytoplasmic,Membrane, Plasma,Membranes, Cell,Membranes, Cytoplasmic,Membranes, Plasma,Plasma Membranes
D002463	Cell Membrane Permeability	A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.	Permeability, Cell Membrane
D004151	Dipeptides	Peptides composed of two amino acid units.	Dipeptide
D006868	Hydrolysis	The process of cleaving a chemical compound by the addition of a molecule of water.
D000596	Amino Acids	Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.	Amino Acid,Acid, Amino,Acids, Amino
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia