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Conventional radioimmunoassays for IgG rheumatoid factors (RF) detect the binding of IgG RF (or their F(ab')2 fragments) to solid-phase human IgG Fc fragments or rabbit IgG. Binding is detected with a radiolabeled antibody that is IgG-specific but is nonreactive with human Fc (i.e., an alpha-Fd reagent) or with rabbit IgG. Anti-Fd reagents are quite laborious to produce. We developed a murine monoclonal alpha-Fd antibody, confirmed its specificity, and compared it with conventional rabbit alpha-Fd in the IgG RF radioimmunoassay. We also adapted the assay to an enzyme-linked assay with both human Fc and rabbit IgG as antigen. We compared the four assay methods with each other and examined their relationship to certain clinical and laboratory features of rheumatoid arthritis.
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
January 1988,
Immunological investigations,
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
March 1994,
Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao,
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
October 2020,
Monoclonal antibodies in immunodiagnosis and immunotherapy,
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
August 1998,
Rheumatic diseases clinics of North America,
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
April 1990,
Clinical and experimental immunology,
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
May 1988,
Journal of immunological methods,
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
January 1979,
Nature,
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
May 1997,
Arthritis and rheumatism,
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
June 2022,
Arthritis & rheumatology (Hoboken, N.J.),
J S McDougal, and
M S Kennedy, and
M Hubbard, and
F C McDuffie, and
D D Moore, and
C H Aloisio, and
D J Phillips, and
R A Roubey, and
C B Reimer
August 1986,
The Journal of experimental medicine,
