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Cyclic adenosine 3':5'-monophosphate (cAMP)-binding proteins and cAMP-dependent protein phosphorylation were examined in growth cone particles (GCPs) prepared from fetal rat brain. Several major proteins which specifically bind a photoactivatable analogue of cAMP are observed in GCPs and correspond to isoelectric variants of the regulatory subunits of the cAMP-dependent protein kinase described in adult brain. We found no evidence for differential compartmentalization of specific cAMP-binding proteins in subcellular fractions of fetal brain or within GCPs. cAMP-stimulated phosphoproteins of GCPs are similar to cAMP-dependent protein kinase substrates characterized in nerve terminals (synaptosomes) of adult brain and include the nerve terminal-specific protein, synapsin I. However, as shown in the companion paper (Katz, F., L. Ellis, and K. H. Pfenninger (1985) J. Neurosci. 5: 1402-1411), this synaptic phosphoprotein is not the major kinase substrate in the GCP fraction. The finding of synapsin I in a subcellular fraction prepared from fetal brain suggests that components of the mature nerve terminal are already present in fetal brain during neuronal sprouting and prior to synaptogenesis.
L Ellis, and
F Katz, and
K H Pfenninger
November 1992,
Molecular endocrinology (Baltimore, Md.),
L Ellis, and
F Katz, and
K H Pfenninger
December 1971,
The Journal of biological chemistry,
L Ellis, and
F Katz, and
K H Pfenninger
October 1973,
Biochimica et biophysica acta,
L Ellis, and
F Katz, and
K H Pfenninger
January 1994,
Journal of neurochemistry,
L Ellis, and
F Katz, and
K H Pfenninger
August 1977,
Proceedings of the National Academy of Sciences of the United States of America,
L Ellis, and
F Katz, and
K H Pfenninger
May 1978,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
L Ellis, and
F Katz, and
K H Pfenninger
January 1976,
Biochemical Society transactions,
L Ellis, and
F Katz, and
K H Pfenninger
May 1983,
The Journal of clinical endocrinology and metabolism,
L Ellis, and
F Katz, and
K H Pfenninger
April 1977,
The Journal of biological chemistry,
L Ellis, and
F Katz, and
K H Pfenninger
November 1976,
The Biochemical journal,
