Biomaterial Database

Repair of indirectly induced DNA damage in human skin fibroblasts treated with N-hydroxy-2-naphthylamine. 1985

M Kaneko, and C Nagata, and M Kodama

The DNA lesions induced by active oxygen species generated from N-hydroxy-2-naphthylamine were quantitated by the alkaline elution technique as single-strand breaks using cultured human-skin fibroblasts. When cells were treated at 20 degrees C for 2 h with 0-25 microM carcinogen, the lesions increased biphasically with the concentration; the increase was slight below 10 microM while it was much larger and dose-dependent above this concentration. The dose response was similar for normal and xeroderma pigmentosum fibroblasts of complementation group A. There was no difference in the repair rate of single-strand breaks formed in these fibroblasts. The rates of repair of single strand breaks induced by N-hydroxy-2-naphthylamine and hydrogen peroxide were similar but slower than that of the repair of gamma-ray-induced single-strand breaks.

UI	MeSH Term	Description	Entries
D009281	Naphthalenes	Two-ring crystalline hydrocarbons isolated from coal tar. They are used as intermediates in chemical synthesis, as insect repellents, fungicides, lubricants, preservatives, and, formerly, as topical antiseptics.
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D004260	DNA Repair	The removal of DNA LESIONS and/or restoration of intact DNA strands without BASE PAIR MISMATCHES, intrastrand or interstrand crosslinks, or discontinuities in the DNA sugar-phosphate backbones.	DNA Damage Response
D004277	DNA, Single-Stranded	A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.	Single-Stranded DNA,DNA, Single Stranded,Single Stranded DNA
D004305	Dose-Response Relationship, Drug	The relationship between the dose of an administered drug and the response of the organism to the drug.	Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005347	Fibroblasts	Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.	Fibroblast
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012867	Skin	The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D014983	Xeroderma Pigmentosum	A rare, pigmentary, and atrophic autosomal recessive disease. It is manifested as an extreme photosensitivity to ULTRAVIOLET RAYS as the result of a deficiency in the enzyme that permits excisional repair of ultraviolet-damaged DNA.	Kaposi Disease,Kaposi's Disease,Kaposis Disease