-transparent.png){kind=logo}
Oocytes from Xenopus laevis microinjected with RNA isolated from Schistosoma mansoni adult worms translated antigens recognized by sera from infected rats, humans, and from immunized rabbits. The pattern of immunoprecipitated proteins analysed by SDS-polyacrylamide gel electrophoresis was species specific in rats. Serum from infected Fischer rats recognized antigens of 20, 27 and several bands in the 50-60 kDa range whereas serum from infected Brown Norway rats also immunoprecipitated major bands at 29, 43 and 100 kDa. Human infection sera gave a very variable pattern of immunoprecipitation not apparently dependent on the patients' age. At least 20 different antigenic species could be identified ranging from 14 to 150 kDa. Some S. mansoni antigenic proteins could be isolated from the membrane fraction of the oocytes whereas notably the 29 kDa band was present mainly in the soluble fraction. N-Glycosylation of S. mansoni antigens occurred as evidenced by the effects of tunicamycin treatment and concanavalin A binding. A multiple series of bands between 50 and 60 kDa, present in the membrane fraction, were glycosylated and secreted from the oocytes. Monoclonal antibodies to larval stage surface antigens failed to immunoprecipitate oocyte translation products, but sera absorbed with live schistosomula identified at least three putative surface antigens of 100, 43 and 29 kDa. However, the 29 kDa molecule was neither synthesized into membranes, nor secreted from oocytes.
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
December 1984,
FEBS letters,
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
October 1982,
International journal for parasitology,
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
July 1992,
The Journal of cell biology,
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
January 1995,
Methods in molecular biology (Clifton, N.J.),
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
April 1986,
Experimental parasitology,
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
January 1982,
Journal of immunological methods,
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
January 1979,
Revista do Instituto de Medicina Tropical de Sao Paulo,
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
January 1998,
Memorias do Instituto Oswaldo Cruz,
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
May 1987,
Developmental biology,
R J Pierce, and
C Aimar, and
J M Balloul, and
M Delarue, and
D Grausz, and
C Verwaerde, and
A Capron
August 1979,
The Journal of parasitology,
