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Rat Tamm-Horsfall glycoprotein (THG) was isolated from urine and disaggregated by gel filtration. Purity was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and autoradiography. Antibodies were raised and used in the development of a specific radioimmunoassay for rat THG. Parallelism could be demonstrated between the standard preparation and urine (diluted to 1:320 with distilled water) and serum (at all dilutions) samples. Recovery of standard added to urine or serum was on average 93% and inter- and intra-assay variation were both below 7%. There was a positive correlation between the mean urinary excretory rate of THG (microgram/24h) and body weight in female rats (p less than 0.001) but not in male rats. Serum THG levels were similar in both sexes and showed a positive correlation with body weight (p less than 0.001; range 43-240 ng/ml). It is concluded that the rat urinary THG excretion rate is similar to that in man and that rat serum also contains a THG-like protein.
P K Wirdnam, and
R D Milner
March 1980,
The Biochemical journal,
P K Wirdnam, and
R D Milner
January 1983,
Journal - Forensic Science Society,
P K Wirdnam, and
R D Milner
June 1974,
Laboratory investigation; a journal of technical methods and pathology,
P K Wirdnam, and
R D Milner
September 1983,
Clinica chimica acta; international journal of clinical chemistry,
P K Wirdnam, and
R D Milner
November 1970,
The Biochemical journal,
P K Wirdnam, and
R D Milner
November 1970,
The Biochemical journal,
P K Wirdnam, and
R D Milner
May 1987,
Nihon Jinzo Gakkai shi,
P K Wirdnam, and
R D Milner
February 1977,
Clinical science and molecular medicine,
P K Wirdnam, and
R D Milner
September 1982,
The Biochemical journal,
P K Wirdnam, and
R D Milner
April 1977,
Molecular and cellular biochemistry,
