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Kupffer and endothelial cells of rat liver were purified by recirculating collagenase perfusion, metrizamide gradient, and counterflow elutriation. Every step of the isolation procedure was followed by light microscopy and histochemistry. The final fractions of purified sinusoidal cells were examined in their isolated state by transmission and scanning electron microscopy. The scope of this manuscript is the detailed documentation and discussion of all technical aspects and problems of a rather new isolation technique, as well as the detailed description of the purified sinusoidal cells of the liver by scanning electron microscopy. Broader acceptance of this isolation procedure should lead to the specific characterization of biochemical and immunological functions of these cells and elucidate their pathophysiological significance.
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
May 1976,
Experimental cell research,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
April 1987,
Nihon Hifuka Gakkai zasshi. The Japanese journal of dermatology,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
August 1984,
The Biochemical journal,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
July 1971,
Experimental cell research,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
September 1980,
Biochemical and biophysical research communications,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
October 1977,
Experimental cell research,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
December 1992,
Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
September 1983,
Journal of immunological methods,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
June 1995,
Experimental hematology,
R N Zahlten, and
H K Hagler, and
M E Nejtek, and
C J Day
April 1993,
Metabolism: clinical and experimental,
