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A method for large-scale production of a pasteurized antithrombin III (AT III) concentrate for therapeutic use has been adapted from published methods. It includes the following steps: (1) batchwise adsorption onto heparin-Sepharose from plasma depleted of cryoprecipitate and prothrombin complex; (2) chromatographic elution at high salt concentration; (3) pasteurization for 10 h at 60 degrees C in the presence of added citrate ion; (4) desalting on Sephadex G-25, and (5) sterile filtration and freeze-drying. Seven batches prepared in this manner gave a mean yield of 269 U AT III/kg plasma. The product passed all the usual animal safety and pyrogenicity tests and has been used successfully in several courses of treatment of congenital deficiencies.
J K Smith, and
L Winkelman, and
D R Evans, and
M E Haddon, and
G Sims
February 1991,
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J K Smith, and
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July 1987,
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J K Smith, and
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J K Smith, and
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May 2014,
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J K Smith, and
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January 1990,
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J K Smith, and
L Winkelman, and
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G Sims
January 1987,
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J K Smith, and
L Winkelman, and
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G Sims
April 2002,
Seminars in thrombosis and hemostasis,
J K Smith, and
L Winkelman, and
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M E Haddon, and
G Sims
January 1990,
Haematologica,
J K Smith, and
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G Sims
August 1990,
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J K Smith, and
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G Sims
September 1989,
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