-transparent.png){kind=logo}
TRH localization in rat brain and hypothalamic tissue was studied by determination of immunoassayable TRH in subcellular fractions prepared from homogenates of these tissues. In both hypothalamus and brain, most of the TRH was found in a crude mitochondrial fraction and its concentration was higher in the synaptosomal fraction isolated by density gradient centrifugation. Electron microscopy was used to verify the composition of the fractions. Exposure of the brain crude mitochondrial fraction to osmotic shock resulted in solubilization of TRH which then was sedimented by centrifugation at 96,000 X g. TRH added to brain homogenates in vitro was rapidly destroyed by incubation at 37 degrees C, but no endogenouse TRH was lost by such incubation. The localization of TRH of hypothalamic and brain tissue to subcellular fractions consisting largely of synaptosomes and synaptic vesicles is compatible with a neurotransmitter function for this substance.
A Winokur, and
R Davis, and
R D Utiger
April 1979,
Brain research,
A Winokur, and
R Davis, and
R D Utiger
August 1984,
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme,
A Winokur, and
R Davis, and
R D Utiger
June 1987,
General and comparative endocrinology,
A Winokur, and
R Davis, and
R D Utiger
January 1982,
Peptides,
A Winokur, and
R Davis, and
R D Utiger
December 1994,
The Journal of comparative neurology,
A Winokur, and
R Davis, and
R D Utiger
January 1986,
Neurochemistry international,
A Winokur, and
R Davis, and
R D Utiger
April 1977,
Endocrinology,
A Winokur, and
R Davis, and
R D Utiger
November 1990,
Endocrinology,
A Winokur, and
R Davis, and
R D Utiger
July 1974,
Science (New York, N.Y.),
A Winokur, and
R Davis, and
R D Utiger
August 1975,
Brain research,
