-transparent.png){kind=logo}
The properties of the cellulosome (the cellulose-binding, multicellulase-containing protein complex) in Clostridium thermocellum were examined by comparing the cellulase systems derived from the wild type and an adherence-defective mutant. The growth conditions--specifically, growth either on cellulose (Avicel) or on cellobiose as insoluble or soluble carbon sources, respectively--were found to be critical to the distribution of the cellulosome in the mutant system: the cellobiose-grown mutant (in contrast to the wild type) lacked the cellulosome on its surface and produced only minor quantities of the extracellular cellulosome accompanied by other relatively low-molecular-weight cellulases. The polypeptide composition of the respective purified cellulosome was dependent on the nature of the carbon source and was similar for both wild-type and mutant cells. Ultrastructural analysis revealed the presence of novel polycellulosomal protuberances on the cell surface of the cellobiose-grown wild type which were absent in the mutant.
E A Bayer, and
E Setter, and
R Lamed
May 1998,
Biochemical Society transactions,
E A Bayer, and
E Setter, and
R Lamed
April 1996,
Biochemistry,
E A Bayer, and
E Setter, and
R Lamed
January 2015,
PloS one,
E A Bayer, and
E Setter, and
R Lamed
December 1998,
Journal of bacteriology,
E A Bayer, and
E Setter, and
R Lamed
February 2012,
Biodegradation,
E A Bayer, and
E Setter, and
R Lamed
April 2011,
Journal of molecular biology,
E A Bayer, and
E Setter, and
R Lamed
June 2002,
Applied and environmental microbiology,
E A Bayer, and
E Setter, and
R Lamed
July 1991,
Journal of bacteriology,
E A Bayer, and
E Setter, and
R Lamed
October 2007,
Journal of bacteriology,
E A Bayer, and
E Setter, and
R Lamed
March 1995,
Journal of bacteriology,
