Biomaterial Database

New acetylcholinesterase-deficient mutants of the nematode Caenorhabditis elegans. 1985

D L Kolson, and R L Russell

We have used visual screening for a mutationally induced uncoordinated phenotype, coupled with subsequent enzymatic assay, to isolate 19 new mutations producing acetylcholinesterase (AChE) deficiencies in the nematode Caenorhabditis elegans. At least 13 of these are independent, and all fall within two genes, ace-1 X and ace-2 I, previously shown to control respectively two kinetically distinct major AChE classes, A and B. Three of the 4 new ace-1 alleles and one of the 15 new ace-2 alleles are apparently leaky; the rest are probably null. For the 3 leaky ace-1 alleles, qualitative changes in the residual class A AChE strongly support the contention that ace-1 is a structural gene. In ace-2;ace-1 mutants, marked reduction in class A and B AChE reveals a third, minor, previously unrecognized AChE class, designated C.

UI	MeSH Term	Description	Entries
D009069	Movement Disorders	Syndromes which feature DYSKINESIAS as a cardinal manifestation of the disease process. Included in this category are degenerative, hereditary, post-infectious, medication-induced, post-inflammatory, and post-traumatic conditions.	Dyskinesia Syndromes,Etat Marbre,Status Marmoratus,Movement Disorder Syndromes,Dyskinesia Syndrome,Movement Disorder,Movement Disorder Syndrome
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D011489	Protein Denaturation	Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.	Denaturation, Protein,Denaturations, Protein,Protein Denaturations
D002107	Caenorhabditis	A genus of small free-living nematodes. Two species, CAENORHABDITIS ELEGANS and C. briggsae are much used in studies of genetics, development, aging, muscle chemistry, and neuroanatomy.	Caenorhabditides
D006358	Hot Temperature	Presence of warmth or heat or a temperature notably higher than an accustomed norm.	Heat,Hot Temperatures,Temperature, Hot,Temperatures, Hot
D000110	Acetylcholinesterase	An enzyme that catalyzes the hydrolysis of ACETYLCHOLINE to CHOLINE and acetate. In the CNS, this enzyme plays a role in the function of peripheral neuromuscular junctions. EC 3.1.1.7.	Acetylcholine Hydrolase,Acetylthiocholinesterase,Hydrolase, Acetylcholine
D000483	Alleles	Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.	Allelomorphs,Allele,Allelomorph
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012995	Solubility	The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)	Solubilities