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A method has been developed for the extraction of proteins from green plant tissues to be used in two-dimensional polyacrylamide gel electrophoresis. Three purification steps were necessary to overcome the problems due to streaking, charge heterogeneity, and other artifacts: After it was ground in liquid nitrogen, the powdered material was stirred in the presence of insoluble polyvinylpyrrolidone for binding phenols, and sodium ascorbate for binding quinones; proteins were precipitated with ammonium sulfate, and the sample was dialyzed. Hundreds of proteins could be detected after Coomassie blue staining using 200 micrograms of proteins from apical buds of Sinapis alba L.
F Cremer, and
C Van de Walle
February 1989,
Analytical biochemistry,
F Cremer, and
C Van de Walle
January 2003,
Electrophoresis,
F Cremer, and
C Van de Walle
May 1984,
Analytical biochemistry,
F Cremer, and
C Van de Walle
January 1974,
Analytical biochemistry,
F Cremer, and
C Van de Walle
January 1984,
Methods in molecular biology (Clifton, N.J.),
F Cremer, and
C Van de Walle
January 1994,
Methods in molecular biology (Clifton, N.J.),
F Cremer, and
C Van de Walle
January 1993,
Methods in molecular biology (Clifton, N.J.),
F Cremer, and
C Van de Walle
January 1983,
Methods in enzymology,
F Cremer, and
C Van de Walle
December 1994,
Molecular biotechnology,
F Cremer, and
C Van de Walle
March 1974,
Plant physiology,
