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After pronase digestion of bovine fibrinogen, the asparagine-linked glycans were released from the resulting glycopeptides by hydrazinolysis, and subsequently re-N-acetylated. Two sialylated glycans were isolated by ion-exchange chromatography. Their primary structure has been determined by methylation analysis and 360-MHz 1H-NMR spectroscopy. The structures proposed to be present in the native glycoprotein are as follows: (formula: see text).
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