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The tetrazolium method for the histochemical detection of monoamine oxidase (MAO) activity in rat liver cryostat sections has been tested for its specificity and its possible use in quantification. The tetrazolium salt tetranitro blue tetrazolium is recommended for the localization of MAO activity, rather than nitro blue tetrazolium or BPST [2-(2-benzothiazolyl)-3-(4-phthalhydrazidyl)-5-styryl-tetrazolium] . Hardly any formazan was produced in the absence of the substrate tryptamine and Marsilid, a specific inhibitor of MAO activity, prevented formazan production almost completely. A linear relationship between the integrated absorbance measured with a microdensitometer and either the incubation period or section thickness was obtained. We conclude that the method described in this paper can be used for the quantitative analysis of MAO activity in tissue sections of rat liver. MAO activity was found to be 20-25% higher in the periportal zone of rat liver than in the perivenous zone.
W M Frederiks, and
F Marx
November 1957,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
W M Frederiks, and
F Marx
September 1963,
American journal of obstetrics and gynecology,
W M Frederiks, and
F Marx
October 1971,
FEBS letters,
W M Frederiks, and
F Marx
January 1967,
Kumamoto Igakkai zasshi. The Journal of the Kumamoto Medical Society,
W M Frederiks, and
F Marx
January 1972,
Acta biologica et medica Germanica,
W M Frederiks, and
F Marx
June 1975,
Histochemistry,
W M Frederiks, and
F Marx
November 1971,
Bollettino della Societa italiana di biologia sperimentale,
W M Frederiks, and
F Marx
October 1978,
Archives of biochemistry and biophysics,
W M Frederiks, and
F Marx
March 1972,
Experientia,
W M Frederiks, and
F Marx
February 1975,
The Biochemical journal,
